{"quality_controlled":"1","publication":"The 1st International Online Conference on Photonics","series_title":"Phys. Sci. Forum","publication_identifier":{"issn":["2673-9984"]},"author":[{"last_name":"Dassmann","first_name":"Nora","full_name":"Dassmann, Nora"},{"first_name":"Bennet","last_name":"Brockhagen","id":"237316","full_name":"Brockhagen, Bennet"},{"first_name":"Andrea","last_name":"Ehrmann","orcid_put_code_url":"https://api.orcid.org/v2.0/0000-0003-0695-3905/work/176200255","full_name":"Ehrmann, Andrea","id":"223776","orcid":"0000-0003-0695-3905"}],"tmp":{"image":"/images/cc_by.png","short":"CC BY (4.0)","name":"Creative Commons Attribution 4.0 International Public License (CC-BY 4.0)","legal_code_url":"https://creativecommons.org/licenses/by/4.0/legalcode"},"file_date_updated":"2025-01-20T14:07:15Z","conference":{"start_date":"2024-10-14","location":"Online","name":"IOCP 2024","end_date":"2024-10-16"},"date_updated":"2025-01-20T15:40:43Z","oa":"1","date_created":"2025-01-20T14:08:03Z","place":"Basel Switzerland","keyword":["electrospinning","poly(acrylonitrile) (PAN)","biopolymers","mammalian cells","fluorescent dyes"],"editor":[{"full_name":"Prudenzano, Francesco ","first_name":"Francesco ","last_name":"Prudenzano"},{"last_name":"Jiang","first_name":"Huabei ","full_name":"Jiang, Huabei "},{"full_name":"Ferrari, Maurizio","first_name":"Maurizio","last_name":"Ferrari"}],"year":"2024","page":"5","publisher":"MDPI","volume":10,"status":"public","type":"conference","urn":"urn:nbn:de:hbz:bi10-53325","has_accepted_license":"1","file":[{"file_size":6858552,"relation":"main_file","file_name":"_2024_Dassmann_PSF10_5.pdf","date_created":"2025-01-20T14:07:15Z","file_id":"5333","creator":"aehrmann","access_level":"open_access","date_updated":"2025-01-20T14:07:15Z","success":1,"content_type":"application/pdf"}],"doi":"10.3390/psf2024010005","intvolume":" 10","publication_status":"published","user_id":"220548","citation":{"ama":"Dassmann N, Brockhagen B, Ehrmann A. Investigation the Optical Contrast Between Nanofiber Mats and Mammalian Cells Dyed with Fluorescent and Other Dyes. In: Prudenzano F, Jiang H, Ferrari M, eds. The 1st International Online Conference on Photonics. Vol 10. Phys. Sci. Forum. Basel Switzerland: MDPI; 2024:5. doi:10.3390/psf2024010005","apa":"Dassmann, N., Brockhagen, B., & Ehrmann, A. (2024). Investigation the Optical Contrast Between Nanofiber Mats and Mammalian Cells Dyed with Fluorescent and Other Dyes. In F. Prudenzano, H. Jiang, & M. Ferrari (Eds.), The 1st International Online Conference on Photonics (Vol. 10, p. 5). Basel Switzerland: MDPI. https://doi.org/10.3390/psf2024010005","alphadin":"Dassmann, Nora ; Brockhagen, Bennet ; Ehrmann, Andrea: Investigation the Optical Contrast Between Nanofiber Mats and Mammalian Cells Dyed with Fluorescent and Other Dyes. In: Prudenzano, F. ; Jiang, H. ; Ferrari, M. (Hrsg.): The 1st International Online Conference on Photonics, Phys. Sci. Forum. Bd. 10. Basel Switzerland : MDPI, 2024, S. 5","ieee":"N. Dassmann, B. Brockhagen, and A. Ehrmann, “Investigation the Optical Contrast Between Nanofiber Mats and Mammalian Cells Dyed with Fluorescent and Other Dyes,” in The 1st International Online Conference on Photonics, Online, 2024, vol. 10, no. 1, p. 5.","mla":"Dassmann, Nora, et al. “Investigation the Optical Contrast Between Nanofiber Mats and Mammalian Cells Dyed with Fluorescent and Other Dyes.” The 1st International Online Conference on Photonics, edited by Francesco Prudenzano et al., vol. 10, no. 1, MDPI, 2024, p. 5, doi:10.3390/psf2024010005.","chicago":"Dassmann, Nora, Bennet Brockhagen, and Andrea Ehrmann. “Investigation the Optical Contrast Between Nanofiber Mats and Mammalian Cells Dyed with Fluorescent and Other Dyes.” In The 1st International Online Conference on Photonics, edited by Francesco Prudenzano, Huabei Jiang, and Maurizio Ferrari, 10:5. Phys. Sci. Forum. Basel Switzerland: MDPI, 2024. https://doi.org/10.3390/psf2024010005.","short":"N. Dassmann, B. Brockhagen, A. Ehrmann, in: F. Prudenzano, H. Jiang, M. Ferrari (Eds.), The 1st International Online Conference on Photonics, MDPI, Basel Switzerland, 2024, p. 5.","bibtex":"@inproceedings{Dassmann_Brockhagen_Ehrmann_2024, place={Basel Switzerland}, series={Phys. Sci. Forum}, title={Investigation the Optical Contrast Between Nanofiber Mats and Mammalian Cells Dyed with Fluorescent and Other Dyes}, volume={10}, DOI={10.3390/psf2024010005}, number={1}, booktitle={The 1st International Online Conference on Photonics}, publisher={MDPI}, author={Dassmann, Nora and Brockhagen, Bennet and Ehrmann, Andrea}, editor={Prudenzano, Francesco and Jiang, Huabei and Ferrari, MaurizioEditors}, year={2024}, pages={5}, collection={Phys. Sci. Forum} }"},"project":[{"_id":"0ec202b7-cd76-11ed-89f4-a9e1a6dbdaa7","name":"Institut für Technische Energie-Systeme"}],"issue":"1","language":[{"iso":"eng"}],"title":"Investigation the Optical Contrast Between Nanofiber Mats and Mammalian Cells Dyed with Fluorescent and Other Dyes","abstract":[{"lang":"eng","text":" Electrospinning can be used to prepare nanofiber mats from diverse polymers and polymer blends. A large area of research is the application of nanofibrous membranes for tissue engineering. Typically, cell adhesion and proliferation as well as the viability of mammalian cells are tested by seeding the cells on substrates, cultivating them for a defined time and finally dyeing them to enable differentiation between cells and substrates under a white light or fluorescence microscope. While this procedure works well for cells cultivated in well plates or petri dishes, other substrates may undesirably also be colored by the dye. Here we show investigations of the optical contrast between dyed CHO DP-12 (Chinese hamster ovary) cells and different electrospun nanofiber mats, dyed with haematoxylin-eosin (H&E), PromoFluor 488 premium, 4,6-diamidino-2-phenylindole (DAPI) or Hoechst 33342, and give the optimum dyeing parameters for maximum optical contrast between cells and nanofibrous substrates."}],"_id":"5332"}